Preparative Low Pressure Liquid Chromatography

COVER

Title

Copyright

Preface

Contents

I Introduction

II Theoretical Part

II.1 Theoretical principles of exclusion chromatography

II.2 Crosslinked dextran gels

III Classification of Chromatography – Separation Mechanisms

IV Column Liquid Chromatographic Separation Systems

IV.1 Column packing materials – Phases

IV.2 Mobile phases – Eluents

IV.3 Preparative columns

IV.4 Equipment

V Practical Part 1 – Preparative LC in Practice

V.1 Sample preparation

V.2 Analytical – Preparative

V.3 Thin-layer chromatography – Sephadex LH-20

V.4 Separation of complex substance mixtures

V.5 Extraction methods – Extraction processes

V.6 Sephadex LH-20 – Separation mechanism A

V.7 Sephadex LH-20 – Separation mechanism B

V.8 Sephadex LH-20 – Scale-up

V.9 Sephadex LH-20 – Loadability

V.10 Sephadex LH-20 compared to …

V.11 Selective preparative separation systems

V.12 Examples of separations from practice

V.13 Glycerides

V.14 Separation of various products

V.15 Separation of depolymerization products

V.16 Enrichment – Quantitation

V.17 Examples of transfers – TLC separation to preparative column

V.18 “Macro” separation – “Mini” production

V.19 Small scale production plant

VI Practical Part 2 – Practice-based Methods for Laboratory Practice

VI.1 Reaction products

VI.2 Extracts

VI.3 Commercial products – Methods of purification

VI.4 Natural products

VI.5 Plant extracts

VI.6 Commercial products

VI.7 Derivatives of PEG oligomers

VI.8 Reaction products

VI.9 Transfer from a TLC plate to a preparative column

VI.10 Mechanism of separations on Sephadex LH-20

VI.11 Plant constituents

VI.12 Cyclodextrins

VI.13 Accomplishable preparative separation proposals

VI.14 Epoxide additions – Separations

VI.15 Isolation of phytosterols, tocopherols, and tocotrienols from enriched samples

VI.16 Pharmaceuticals

VI.17 Depolymerization products

Appendix

A.1 Reaction products – Separations on Sephadex LH-20

A.2 Miscellaneous separations from everyday practice

A.3 Test mixtures and separation mechanisms on Sephadex LH-20 Molecular structures and elution behavior

Remarks

Glossary

Dictionary of chromatography

Company directory

Bibliography

Index